Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.
Identifieur interne : 001003 ( Main/Exploration ); précédent : 001002; suivant : 001004Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.
Auteurs : Alexia Daoust [France] ; Emmanuel Barbier [France] ; Sylvain Bohic [France]Source :
- NeuroImage [ 1053-8119 ] ; 2012-09-17.
Abstract
Manganese enhanced MRI (MEMRI) offers many possibilities such as tract tracing and functional imaging in vivo. Mn is however neurotoxic and may induce symptoms similar to those associated with Parkinson's disease (manganism). The mechanisms of Mn-induced neurotoxicity are not clear. In this study, we combine synchrotron X-ray fluorescence microprobe (SR-XRF) and MEMRI techniques to investigate spatial distribution of Mn within the rat hippocampus and how Mn interacts with Ca, Fe and Zn at a cellular level. Images were acquired in the rat hippocampus (n=23) and using two injection routes: intra-cerebral (MnCl(2): 50mM, 10μL) and intra-peritoneal (MnCl(2): 100mM, 30mg/kg). For both injection routes, Mn is found in dentate gyrus and in CA3: control: 2.5±1.6, intra-peritoneal: 5.0±2.4, and intra-cerebral: 25.1±9.2μg/g. Mn follows Zn distribution and has a negative impact on the total amount of Zn and Fe. The Mn-enhanced MRI contrast is well correlated with the total Mn amount measured with SR-XRF (R(2)=0.93; p<0.002). After intra-cerebral injection, the hippocampal fissure is found to accumulate a large amount of Mn and yields a hypointense MRI signal, which may be ascribed to a reduction in T2. This study shows that SR-XRF is well suited to investigate Mn distribution at a mesoscale and that MRI is sensitive to low Mn concentrations. As perturbations in metal homeostasis may alter brain function, the injected dose of Mn in MEMRI studies needs to be carefully adjusted to obtain reliable functional information.
Url:
DOI: 10.1016/j.neuroimage.2012.09.025
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Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9</addrLine> <country key="FR"></country> </address> <ref type="url">http://neurosciences.ujf-grenoble.fr/</ref> </desc> <listRelation> <relation active="#struct-51016" type="direct"></relation> <relation name="DSV/IRTSV" active="#struct-300016" type="direct"></relation> <relation active="#struct-300071" type="direct"></relation> <relation name="U836" active="#struct-303623" type="direct"></relation> </listRelation></org></tutelle><tutelle active="#struct-51016" type="indirect"><org type="institution" xml:id="struct-51016" status="OLD"> <idno type="IdRef">026404796</idno> <orgName>Université Joseph Fourier - Grenoble 1</orgName> <orgName type="acronym">UJF</orgName> <date type="end">2015-12-31</date> <desc> <address> <addrLine>BP 53 - 38041 Grenoble Cedex 9</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ujf-grenoble.fr/</ref> </desc></org></tutelle><tutelle name="DSV/IRTSV" active="#struct-300016" type="indirect"><org type="institution" xml:id="struct-300016" status="VALID"> <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName> <orgName type="acronym">CEA</orgName> <desc> <address> <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.cea.fr/</ref> </desc></org></tutelle><tutelle active="#struct-300071" type="indirect"><org type="institution" xml:id="struct-300071" status="VALID"> <orgName>CHU Grenoble</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle><tutelle name="U836" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc></org></tutelle><tutelle active="#struct-2568" type="direct"><org type="institution" xml:id="struct-2568" status="VALID"> <orgName>European Synchrotron Radiation Facility</orgName> <orgName type="acronym">ESRF</orgName> <desc> <address> <addrLine>6 rue Jules Horowitz BP220 38043 GRENOBLE CEDEX</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.esrf.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Grenoble</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Joseph Fourier</orgName><orgName type="institution" wicri:auto="newGroup">Université de Grenoble</orgName></affiliation></author></analytic><idno type="DOI">10.1016/j.neuroimage.2012.09.025</idno><series><title level="j">NeuroImage</title><idno type="ISSN">1053-8119</idno><imprint><date type="datePub">2012-09-17</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="id"><term>MEMRI</term><term>MRI</term><term>Manganese</term><term>X-ray synchrotron</term><term>hippocampus</term><term>rat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Manganese enhanced MRI (MEMRI) offers many possibilities such as tract tracing and functional imaging in vivo. Mn is however neurotoxic and may induce symptoms similar to those associated with Parkinson's disease (manganism). The mechanisms of Mn-induced neurotoxicity are not clear. In this study, we combine synchrotron X-ray fluorescence microprobe (SR-XRF) and MEMRI techniques to investigate spatial distribution of Mn within the rat hippocampus and how Mn interacts with Ca, Fe and Zn at a cellular level. Images were acquired in the rat hippocampus (n=23) and using two injection routes: intra-cerebral (MnCl(2): 50mM, 10μL) and intra-peritoneal (MnCl(2): 100mM, 30mg/kg). For both injection routes, Mn is found in dentate gyrus and in CA3: control: 2.5±1.6, intra-peritoneal: 5.0±2.4, and intra-cerebral: 25.1±9.2μg/g. Mn follows Zn distribution and has a negative impact on the total amount of Zn and Fe. The Mn-enhanced MRI contrast is well correlated with the total Mn amount measured with SR-XRF (R(2)=0.93; p<0.002). After intra-cerebral injection, the hippocampal fissure is found to accumulate a large amount of Mn and yields a hypointense MRI signal, which may be ascribed to a reduction in T2. This study shows that SR-XRF is well suited to investigate Mn distribution at a mesoscale and that MRI is sensitive to low Mn concentrations. As perturbations in metal homeostasis may alter brain function, the injected dose of Mn in MEMRI studies needs to be carefully adjusted to obtain reliable functional information.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li></settlement><orgName><li>Université Joseph Fourier</li><li>Université de Grenoble</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Daoust, Alexia" sort="Daoust, Alexia" uniqKey="Daoust A" first="Alexia" last="Daoust">Alexia Daoust</name></region><name sortKey="Barbier, Emmanuel" sort="Barbier, Emmanuel" uniqKey="Barbier E" first="Emmanuel" last="Barbier">Emmanuel Barbier</name><name sortKey="Bohic, Sylvain" sort="Bohic, Sylvain" uniqKey="Bohic S" first="Sylvain" last="Bohic">Sylvain Bohic</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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